JOURNAL OF PHARMACOLOGY AND
BIOMEDICINE
ISSN.2456-8244
The prime objective of this work was to formulate selenium nano- particles, surface decorate them with phenyl alanine to impart colon tar- geting ability and load the particles with mesalamine. The colon targeted selenium nanoparticles were prepared by placing phenyl alanine on the surface of the selenium nanoparticles and then loading mesalamine on the surface of PA-d-SeNPs to produce PA-Se@Mes nanoparticles. The characteristic peaks of selenium nanoparticles (3555.92 for OH stretch, 3107.48 & 2943.50 for CH stretch, C-C stretch at 1465.96) were present in PA-d-SeNPs also. Additionally, a characteristic amide linkage (1712.86) was also visible confirming surface attachment of phenyl alanine. The colorimetric estimation revealed an attachment of 69.1% phenyl alanine on the surface of the nanoparticles. The yield of the nanoparticles was found to be in the range of 60-65%. The average drug loading was found to be 14.6 ± 0.21% whereas the average encapsulation efficiency was found to be 31.8 ± 0.37 %. The average particle size of selenium nanopar- ticles, PA-d-SeNPs and PA-Se@Mes was found to be 212.7, 140.6, 104.2 nm respectively. The zeta potential of the PA-Se@Mes was found to be - 16.0 ± 3.70 mV. It was found that 85.19% mesalamine was released from PA-Se@Mes in presence of rat caecal content after 24 hours while only 69.31% was released in absence of rat caecal content, suggesting colon targeting.
NAMES:
ONLINE ISSN:2456-8244
Keywords: Selenium , Mesalamine , Nanoparticles , Targeting , Colon
DOI:
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